Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary TargetNitric oxide (NO) fluorescent indicator

Cell permeable: yes

General description

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

Legal Information

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

In DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Warning

Toxicity: Irritant (B)